报告题目：Genome targeting with programmable nucleases in animal model generation and beyond

报 告  人：黄行许 教授

南京大学模式动物研究所 PI

报告时间：7月18日上午9:00-11:00

报告地点：闵行校区生物药学楼1-105室

联 系  人：吴雨泽   This e-mail address is being protected from spambots. You need JavaScript enabled to view it.

 

研究方向：

1、建立不同基因工程小鼠模型研究配子发生的调控；

2、结合利用同源重组技术、锌指蛋白酶技术、RNA干扰技术、iPS技术进行不同动物的基因组改造。

代表性论文：

1. Wang L, Zhang J, Duan J, Gao X, Zhu W, Song C, Yang L, Zhang J, Li G, Ci W, Li W, Zhou Q, Tang F, He C, Huang X*, Liu J*. Programming and inheritance of parental DNA methylome in mammal. Cell 2014, 157(4): 979-991.

2. Shen B, Zhang J, Zhang W, Zhou J, Wang J, Chen L, Wang L, Hogkins A, Iyer V, Huang X*, Skarnes W*. Efficient genome modification in mice by CRISPR/Cas9 nickase without off-target effects. Nature Methods 2014, 11(4): 399-402.

3. Niu Y, Shen B, Cui Y, Chen Y, Wang J, Wang L, Kang Y, Zhao X, Si W, Li W, Xiang AP, Zhou J, Guo X, Si C, Hu B, Dong G, Wang H, Zhou Z, Li T, Tan T, Pu X, Wang F, Ji S, Zhou Q, Huang X*, Ji W*, Sha J*. Generation of gene-modified cynomolgus monkey via Cas9/RNA-mediated gene targeting in one-cell embryos. Cell 2014, 156(4): 836-843.

4. Ma Y, Zhang X, Shen B, Lu Y, Chen W, Ma J, Bai L, Huang X*, Zhang L*. Generating rats with conditional alleles using CRISPR/Cas9. Cell Research 2014, 24(1): 122-125.

5. Shen B, Zhang J, Wu H, Wang J, Ma K, Li Z, Zhang X, Zhang P, Huang X*. Mouse gene knockout by Cas9/RNA-mediated site-specific gene targeting. Cell Research 2013, 23(5): 720-723.